Proteintech, 13471-1-AP, SDCCAG8 Polyclonal antibody

Size: 20ul / 150ul The SDCCAG8 (13471-1-AP) by Proteintech is a Polyclonal antibody targeting SDCCAG8 in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, canine, zebrafish samples 13471-1-AP targets SDCCAG8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine, zebrafish samples. Tested Applications Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms. Specification Tested Reactivity: human, mouse, rat, canine, zebrafish Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4264 Product name: Recombinant human SDCCAG8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC032454 Sequence: MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQSHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCKEELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDNADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEANFEKTKHPSQ Predict reactive species Full Name: serologically defined colon cancer antigen 8 Calculated Molecular Weight: 713 aa, 83 kDa Observed Molecular Weight: 83 kDa GenBank Accession Number: BC032454 Gene Symbol: SDCCAG8 Gene ID (NCBI): 10806 RRID: AB_2183417 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86SQ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.