Proteintech, 13498-1-AP, RUFY1 Polyclonal antibody

Size: 20ul / 150ul The RUFY1 (13498-1-AP) by Proteintech is a Polyclonal antibody targeting RUFY1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13498-1-AP targets RUFY1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4324 Product name: Recombinant human RUFY1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 219-600 aa of BC032571 Sequence: TKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS Predict reactive species Full Name: RUN and FYVE domain containing 1 Calculated Molecular Weight: 708 aa, 80 kDa Observed Molecular Weight: 69 kDa, 80 kDa GenBank Accession Number: BC032571 Gene Symbol: RUFY1 Gene ID (NCBI): 80230 RRID: AB_2183747 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96T51 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.