Proteintech, 13510-1-AP, eEF2K Polyclonal antibody

Size: 20ul / 150ul The eEF2K (13510-1-AP) by Proteintech is a Polyclonal antibody targeting eEF2K in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 13510-1-AP targets eEF2K in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, Jurkat cells, HEK-293 cells Positive IP detected in: PC-3 cells Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Specification Tested Reactivity: human Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4432 Product name: Recombinant human EEF2K protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 376-725 aa of BC032665 Sequence: LSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE Predict reactive species Full Name: eukaryotic elongation factor-2 kinase Calculated Molecular Weight: 725 aa, 100 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC032665 Gene Symbol: EEF2K Gene ID (NCBI): 29904 RRID: AB_2277761 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.