Proteintech, 13511-1-AP, Beta-2-Microglobulin Polyclonal antibody

Size: 20ul / 150ul Beta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA 13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, Hepa1-6 cells, human heart tissue, human stomach tissue, mouse lung tissue, HeLa cells, HepG2 cells, Jurkat cells, Raji cells, mouse spleen tissue, rat spleen tissue, rat lung tissue Positive IP detected in: A431 cells Positive IHC detected in: human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, NCCIT cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:375-1:1500 Background Information Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4433 Product name: Recombinant human B2M protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-119 aa of BC032589 Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Predict reactive species Full Name: beta-2-microglobulin Calculated Molecular Weight: 119 aa, 14 kDa Observed Molecular Weight: 12-14 kDa GenBank Accession Number: BC032589 Gene Symbol: B2M Gene ID (NCBI): 567 ENSEMBL Gene ID: ENSG00000166710 RRID: AB_2062735 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P61769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.