Proteintech, 13571-1-AP, ARFGAP1 Polyclonal antibody

Size: 20ul / 150ul The ARFGAP1 (13571-1-AP) by Proteintech is a Polyclonal antibody targeting ARFGAP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13571-1-AP targets ARFGAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, human brain tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4468 Product name: Recombinant human ARFGAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-414 aa of BC028233 Sequence: MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW Predict reactive species Full Name: ADP-ribosylation factor GTPase activating protein 1 Calculated Molecular Weight: 414 aa, 46 kDa Observed Molecular Weight: 46 kDa, 50 kDa GenBank Accession Number: BC028233 Gene Symbol: ARFGAP1 Gene ID (NCBI): 55738 RRID: AB_2058621 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N6T3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.