Proteintech, 13624-1-AP, CAMKK1 Polyclonal antibody

Size: 20ul / 150ul The CAMKK1 (13624-1-AP) by Proteintech is a Polyclonal antibody targeting CAMKK1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 . Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4534 Product name: Recombinant human CAMKK1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-348 aa of BC031647 Sequence: MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSST Predict reactive species Full Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha Calculated Molecular Weight: 520 aa, 57 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC031647 Gene Symbol: CAMKK1 Gene ID (NCBI): 84254 RRID: AB_2068403 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N5S9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.