Proteintech, 13658-1-AP, NR2C1 Polyclonal antibody

Size: 20ul / 150ul The NR2C1 (13658-1-AP) by Proteintech is a Polyclonal antibody targeting NR2C1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13658-1-AP targets NR2C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, PC-3 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4554 Product name: Recombinant human NR2C1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 118-467 aa of BC040141 Sequence: DKASGRHYGAVTCEGCKGFFKRSIRKNLVYSCRGSKDCIINKHHRNRCQYCRLQRCIAFGMKQDSVQCERKPIEVSREKSSNCAASTEKIYIRKDLRSPLTATPTFVTDSESTRSTGLLDSGMFMNIHPSGVKTESAVLMTSDKAESCQGDLSTLANVVTSLANLGKTKDLSQNSNEMSMIESLSNDDTSLCEFQEMQTNGDVSRAFDTLAKALNPGESTACQSSVAGMEGSVHLITGDSSINYTEKEGPLLSDSHVAFRLTMPSPMPEYLNVHYIGESASRLLFLSMHWALSIPSFQALGQENSISLVKAYWNELFTLGLAQCWQVMNVATILATFVNCLHNSLQQAEG Predict reactive species Full Name: nuclear receptor subfamily 2, group C, member 1 Calculated Molecular Weight: 467 aa, 52 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC040141 Gene Symbol: NR2C1 Gene ID (NCBI): 7181 RRID: AB_2236290 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13056 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.