Proteintech, 13734-1-AP, TFF1 Polyclonal antibody

Size: 20ul / 150ul The TFF1 (13734-1-AP) by Proteintech is a Polyclonal antibody targeting TFF1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, human kidney tissue, mouse stomach tisssue Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human ovary tumor tissue, human stomach tissue, mouse stomach tissue, rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4541 Product name: Recombinant human TFF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC032811 Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF Predict reactive species Full Name: trefoil factor 1 Calculated Molecular Weight: 84 aa, 9 kDa Observed Molecular Weight: 9-12 kDa GenBank Accession Number: BC032811 Gene Symbol: TFF1 Gene ID (NCBI): 7031 RRID: AB_2202853 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04155 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.