Proteintech, 13744-1-AP, TRIM22 Polyclonal antibody

Size: 20ul / 150ul The TRIM22 (13744-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM22 in IHC, ELISA applications with reactivity to human samples 13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human lung cancer tissue, mouse spleen tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid cells (PMID: 32814880). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4683 Product name: Recombinant human TRIM22 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC035582 Sequence: MDFSVKVDIEKEVTCPICLELLTEPLSLDCGHSFCQACITAKIKESVIISRGESSCPVCQTRFQPGNLRPNRHLANIVERVKEVKMSPQEGQKRDVCEHHGKKLQIFCKEDGKVICWVCELSQEHQGHQTFRINEVVKECQEKLQVALQRLIKEDQEAEKLEDDIRQERTAWKNYIQIERQKILKGFNEMRVILDNEEQRELQKLEEGEVNVLDNLAAATDQLVQQRQDASTLISDLQRRLRGSSVEMLQDVIDVMKRSESWTLKKPKSVSKKLKSVFRVPDLSGMLQVLKELTDVQYYWVDVMLNPGSATSNVAISVDQRQVKTVRTCTFKNSNPCDFSAFGVFGCQYF Predict reactive species Full Name: tripartite motif-containing 22 Calculated Molecular Weight: 498 aa, 57 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC035582 Gene Symbol: TRIM22 Gene ID (NCBI): 10346 RRID: AB_2877974 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IYM9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.