Proteintech, 13751-1-AP, MYST2 Polyclonal antibody

Size: 20ul / 150ul The MYST2 (13751-1-AP) by Proteintech is a Polyclonal antibody targeting MYST2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13751-1-AP targets MYST2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells Positive IP detected in: HeLa cells Positive IHC detected in: human pancreas cancer tissue, human thyroid cancer tissue, mouse stomach tissue, rat stomach tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4693 Product name: Recombinant human MYST2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC032640 Sequence: MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEF Predict reactive species Full Name: MYST histone acetyltransferase 2 Calculated Molecular Weight: 611 aa, 71 kDa Observed Molecular Weight: 71 kDa GenBank Accession Number: BC032640 Gene Symbol: MYST2 Gene ID (NCBI): 11143 RRID: AB_2266703 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95251 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.