Proteintech, 13761-1-AP, CDK8/19 Polyclonal antibody

Size: 20ul / 150ul The CDK8/19 (13761-1-AP) by Proteintech is a Polyclonal antibody targeting CDK8/19 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13761-1-AP targets CDK8/19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Y79 cells, mouse brain tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:300-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4731 Product name: Recombinant human CDC2L6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-335 aa of BC037289 Sequence: MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYF Predict reactive species Full Name: cell division cycle 2-like 6 (CDK8-like) Calculated Molecular Weight: 502 aa, 57 kDa Observed Molecular Weight: 50-57 kDa GenBank Accession Number: BC037289 Gene Symbol: CDK19 Gene ID (NCBI): 23097 RRID: AB_2276048 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BWU1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.