Proteintech, 13762-1-AP, AMBRA1 Polyclonal antibody

Size: 20ul / 150ul The AMBRA1 (13762-1-AP) by Proteintech is a Polyclonal antibody targeting AMBRA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, HepG2 cells, HT-1080 cells, human brain tissue, mouse brain tissue, U2OS cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application. The full length of Ambra1 is about 140 kDa, with the C-terminal fragment measuring approximately 100 kDa and the N-terminal fragment measuring approximately 50 kDa (PMID: 22441670). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4735 Product name: Recombinant human AMBRA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 906-1208 aa of BC045609 Sequence: NVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTETLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTVHCELTNNNHLLDGGSSRGDAAGPRGEPRNR Predict reactive species Full Name: autophagy/beclin-1 regulator 1 Calculated Molecular Weight: 1208 aa, 133 kDa Observed Molecular Weight: 110kDa, 130-135 kDa GenBank Accession Number: BC045609 Gene Symbol: AMBRA1 Gene ID (NCBI): 55626 RRID: AB_10642007 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9C0C7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.