Proteintech, 13934-1-AP, FBXL14 Polyclonal antibody

Size: 20ul / 150ul The FBXL14 (13934-1-AP) by Proteintech is a Polyclonal antibody targeting FBXL14 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 13934-1-AP targets FBXL14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4975 Product name: Recombinant human FBXL14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-418 aa of BC028132 Sequence: METHISCLFPELLAMIFGYLDVRDKGRAAQVCTAWRDAAYHKSVWRGVEAKLHLRRANPSLFPSLQARGIRRVQILSLRRSLSYVIQGMANIESLNLSGCYNLTDNGLGHAFVQEIGSLRALNLSLCKQITDSSLGRIAQYLKGLEVLELGGCSNITNTGLLLIAWGLQRLKSLNLRSCRHLSDVGIGHLAGMTRSAAEGCLGLEQLTLQDCQKLTDLSLKHISRGLTGLRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFCDKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLELIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTDSEKEARGDFSPLFTVRTRGSSRR Predict reactive species Full Name: F-box and leucine-rich repeat protein 14 Calculated Molecular Weight: 418 aa, 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC028132 Gene Symbol: FBXL14 Gene ID (NCBI): 144699 RRID: AB_10547838 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N1E6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.