Proteintech, 13944-1-AP, WARS2 Polyclonal antibody

Size: 20ul / 150ul The WARS2 (13944-1-AP) by Proteintech is a Polyclonal antibody targeting WARS2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13944-1-AP targets WARS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human liver tissue, mouse brain tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information WARS2(tryptophan--tRNA ligase, mitochondrial) is also called mitochondrial TRPRS and belongs to the class-I aminoacyl-tRNA synthetase family. The gene encodes the protein containing 360-amino acid ,which has an N-terminal mitochondrial targeting signal that is cleaved after residue 18. WARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold(PMID:15779907). It has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5024 Product name: Recombinant human WARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC044575 Sequence: MALHSMRKARERWSFIRALHKGSAAAPALQKDSKKRVFSGIQPTGILHLSNYLGAIESWVRLQDEYDSVLYSIVDLHSITVPQDPAVLRQSILDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLHQWKAKTTKQKHDGTVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGFNKKYGEFFPVPESILTSMKKVKSLRDPSAKMSKSDPDKLATVRITDSPEEIVQKFRKAVTDFTSEVTYDPAGRAGVSNIVAVHAAVTGLSVEEVVRRSAGMNTARYKLAVADAVIEKFAPIKREIEKLKLDKDHLEKVLQIGSAKAKELAYTVCQEVKKLVGFL Predict reactive species Full Name: tryptophanyl tRNA synthetase 2, mitochondrial Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 60 kDa/55 kDa GenBank Accession Number: BC044575 Gene Symbol: WARS2 Gene ID (NCBI): 10352 RRID: AB_2304541 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UGM6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.