Proteintech, 13976-1-AP, CNNM3 Polyclonal antibody

Size: 20ul / 150ul The CNNM3 (13976-1-AP) by Proteintech is a Polyclonal antibody targeting CNNM3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13976-1-AP targets CNNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HepG2 cells, K-562 cells, Raji cells Positive IP detected in: K-562 cells Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Human Cyclin M 3 (CNNM3) has five transmembrane domains located at the N-terminal region and a C terminus containing a CBS pair domain and a cyclin box, both of which are part of the ACD region. CNNM3 magnesium transporter raises the intracellular magnesium content of cancer cells contributing to increased tumor growth and the acquisition of oncogenic properties (PMID: 24632616). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5012 Product name: Recombinant human CNNM3 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 358-707 aa of BC037272 Sequence: NIVDMLYLKDLAFVDPEDCTPLSTITRFYNHPLHFVFNDTKLDAVLEEFKRGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIRSEILDESEDYRDTVVKRKPASLMAPLKRKEEFSLFKVSDDEYKVTISPQLLLATQRFLSREVDVFSPLRISEKVLLHLLKHPSVNQEVRFDESNRLATHHYLYQRSQPVDYFILILQGRVEVEIGKEGLKFENGAFTYYGVSALTVPSSVHQSPVSSLQPIRHDLQPDPGDGTHSSAYCPDYTVRALSDLQLIKVTRLQYLNALLATRAQNLPQSPENTDLQVIPGSQTRLLGEKTTTAAGSSHSRPGVPVEGSPGRNPGV Predict reactive species Full Name: cyclin M3 Calculated Molecular Weight: 76 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC037272 Gene Symbol: CNNM3 Gene ID (NCBI): 26505 RRID: AB_2082153 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NE01 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.