Proteintech, 13979-1-AP, TLK2 Polyclonal antibody

Size: 20ul / 150ul The TLK2 (13979-1-AP) by Proteintech is a Polyclonal antibody targeting TLK2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 13979-1-AP targets TLK2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5022 Product name: Recombinant human TLK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 512-831 aa of BC044925 Sequence: DHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN Predict reactive species Full Name: tousled-like kinase 2 Calculated Molecular Weight: 772 aa, 88 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC044925 Gene Symbol: TLK2 Gene ID (NCBI): 11011 RRID: AB_2203887 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86UE8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.