Proteintech, 14084-1-AP, CNKSR3 Polyclonal antibody

Size: 20ul / 150ul The CNKSR3 (14084-1-AP) by Proteintech is a Polyclonal antibody targeting CNKSR3 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 14084-1-AP targets CNKSR3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human brain tissue, mouse brain tissue Positive IP detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Connector enhancer of kinase suppressor of ras 3 (CNKSR3) gene encodes CNK homolog protein 3 (CNK3). CNK proteins, originally identified in Drosophila, were described to regulate the RAS/ERK pathway at the level of the RAS protein kinase. CNKSR3, homologue of scaffold proteins involved in MAPK pathway regulation, is a direct target of mineralocorticoid receptor (MR). CNK3 coordinates the assembly of a multiprotein epithelial sodium channel (ENaC) -regulatory complex, and hence plays a central role in sodium homeostasis. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5213 Product name: Recombinant human CNKSR3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC060761 Sequence: MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLCALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDRAPFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLEEVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRENPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPPAV Predict reactive species Full Name: CNKSR family member 3 Calculated Molecular Weight: 555 aa, 62 kDa Observed Molecular Weight: 62-65 kDa GenBank Accession Number: BC060761 Gene Symbol: CNKSR3 Gene ID (NCBI): 154043 RRID: AB_2229651 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6P9H4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.