Proteintech, 14099-1-AP, ASAP3 Polyclonal antibody

Size: 20ul / 150ul The ASAP3 (14099-1-AP) by Proteintech is a Polyclonal antibody targeting ASAP3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 14099-1-AP targets ASAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, L02 cells Positive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5231 Product name: Recombinant human ASAP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 558-903 aa of BC060786 Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD Predict reactive species Full Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 Calculated Molecular Weight: 903 aa, 99 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC060786 Gene Symbol: ASAP3 Gene ID (NCBI): 55616 RRID: AB_2059021 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TDY4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.