Proteintech, 14138-1-AP, PTPRF Polyclonal antibody

Size: 20ul / 150ul The PTPRF (14138-1-AP) by Proteintech is a Polyclonal antibody targeting PTPRF in WB, ELISA applications with reactivity to human samples 14138-1-AP targets PTPRF in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, HeLa cells, HepG2 cells, MKN-45 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information PTPRF (Protein Tyrosine Phosphatase Receptor Type F) is a receptor protein tyrosine phosphatase, also known as LAR. PTPRF is a transmembrane protein with extracellular domain, transmembrane domain and two intracellular catalytic domains in series. It is located in the cell membrane and participates in the interaction between cells or cell matrix. It is widely expressed in many tissues, including fat, skin, heart, lung, liver, kidney, pancreas, small intestine, colon, brain, skeletal muscle, spleen, peripheral white blood cells and so on. The protein plays an important role in regulating a variety of cell processes, including cell growth, differentiation, mitotic cycle and carcinogenic transformation. In the insulin-responsive tissues of obese and insulin-resistant individuals, the expression level of PTPRF is increased, which may contribute to the pathogenesis of insulin resistance. PTPRF showed expression changes in many cancers, such as breast cancer, thyroid cancer, non-small cell lung cancer and so on. In gastric adenocarcinoma, PTPRF, as a new tumor suppressor, plays a role by inactivating ERK1/2 signaling pathway (PMID: 32973331). The total length of the protein is 175-200kd, and after cutting, it forms 125-150 and 80-85kd,70 and 72kDa fragments (PMID: 1547787, PMID: 17259169). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5374 Product name: Recombinant human PTPRF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1549-1898 aa of BC048768 Sequence: FIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, F Calculated Molecular Weight: 1898 aa, 212 kDa Observed Molecular Weight: 175 kDa-200kDa GenBank Accession Number: BC048768 Gene Symbol: PTPRF Gene ID (NCBI): 5792 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P10586 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.