Proteintech, 14237-1-AP, DUSP16 Polyclonal antibody

Size: 20ul / 150ul The DUSP16 (14237-1-AP) by Proteintech is a Polyclonal antibody targeting DUSP16 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 14237-1-AP targets DUSP16 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-12 cells, COLO 320 cells, HeLa cells, mouse brain tissue Positive IHC detected in: human colon tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5479 Product name: Recombinant human DUSP16 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 313-662 aa of BC042101 Sequence: LLHLEKPNEPVPAVSEGGQKSETPLSPPCADSATSEAAGQRPVHPASVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSFSLDIKSVSYSASMAASLHGFSSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQSKRLHSVRTSSSGTAQRSLLSPLHRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFATESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQMEFGESIMSENRSREELGKVGSQSSFSGSMEIIEVS Predict reactive species Full Name: dual specificity phosphatase 16 Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC042101 Gene Symbol: DUSP16 Gene ID (NCBI): 80824 RRID: AB_2878031 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BY84 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.