Proteintech, 14238-1-AP, Myocilin Polyclonal antibody

Size: 20ul / 150ul The Myocilin (14238-1-AP) by Proteintech is a Polyclonal antibody targeting Myocilin in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 14238-1-AP targets Myocilin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, human stomach tissue, mouse skeletal muscle tissue Positive IP detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5492 Product name: Recombinant human MYOC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 154-504 aa of BC029261 Sequence: EEKKRLRQENENLARRLESSSQEVARLRRGQCPQTRDTARAVPPGSREVSTWNLDTLAFQELKSELTEVPASRILKESPSGYLRSGEGDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPYTQETTWRIDTVGTDVRQVFEYDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELNTETVKAEKEIPGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETNIRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYNPLEKKLFAWDNLNMVTYDIKLSKM Predict reactive species Full Name: myocilin, trabecular meshwork inducible glucocorticoid response Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC029261 Gene Symbol: Myocilin Gene ID (NCBI): 4653 RRID: AB_2148652 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.