Proteintech, 14245-1-AP, BIN2 Polyclonal antibody

Size: 20ul / 150ul The BIN2 (14245-1-AP) by Proteintech is a Polyclonal antibody targeting BIN2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, U-937 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human spleen tissue, mouse heart tissue, human heart tissue, human skin tissue, human lung tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:14000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846) Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5502 Product name: Recombinant human BIN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 216-565 aa of BC047686 Sequence: LRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISADSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL Predict reactive species Full Name: bridging integrator 2 Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 80-90 kDa GenBank Accession Number: BC047686 Gene Symbol: BIN2 Gene ID (NCBI): 51411 RRID: AB_2063647 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBW5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.