Proteintech, 14315-1-AP, SLC38A3 Polyclonal antibody

Size: 20ul / 150ul The SLC38A3 (14315-1-AP) by Proteintech is a Polyclonal antibody targeting SLC38A3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 14315-1-AP targets SLC38A3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse liver tissue, mouse pancreas tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5553 Product name: Recombinant human SLC38A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC042875 Sequence: MEAPLQTEMVELVPNGKHSEGLLPVITPMAGNQRVEDPARSCMEGKSFLQKSPSKEPHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHLLLKSSGVVGIRAYEQLGYRAFGTPGKLAAALAITLQNIGAMSSYLYIIKSELPLVIQTFLNLEEKTSDWYMNGNY Predict reactive species Full Name: solute carrier family 38, member 3 Calculated Molecular Weight: 504 aa, 56 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC042875 Gene Symbol: SLC38A3 Gene ID (NCBI): 10991 RRID: AB_2254879 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99624 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.