Proteintech, 14372-1-AP, MTMR2 Polyclonal antibody

Size: 20ul / 150ul The MTMR2 (14372-1-AP) by Proteintech is a Polyclonal antibody targeting MTMR2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human cerebellum tissue, NIH/3T3 cells Positive IP detected in: mouse cerebellum tissue Positive IHC detected in: human endometrial cancer tissue, human cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5745 Product name: Recombinant human MTMR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 294-643 aa of BC052990 Sequence: KRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVFLDIHNIHVMRESLRKLKEIVYPNIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWDRTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQMTRQFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKRTVSLWSYINSQLEDFTNPLYGSYSNHVLYPVASMRHLELWVGYYIRWNPRMKPQEPIHNRYKELLAKRAELQKKVEELQREISNRSTSSSERASSPAQCVTPVQTVV Predict reactive species Full Name: myotubularin related protein 2 Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 66-73 kDa GenBank Accession Number: BC052990 Gene Symbol: MTMR2 Gene ID (NCBI): 8898 RRID: AB_2282336 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13614 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.