Proteintech, 14379-1-AP, PON2 Polyclonal antibody

Size: 20ul / 150ul The PON2 (14379-1-AP) by Proteintech is a Polyclonal antibody targeting PON2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 14379-1-AP targets PON2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human liver tissue, L02 cells Positive IP detected in: L02 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5759 Product name: Recombinant human PON2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC046160 Sequence: MGRLVAVGLLGIALALLGERLLALRNRLKASREVESVDLPHCHLIKGIEAGSEDIDILPNGLAFFSVGLKFPGLHSFAPDKPGGILMMDLKEEKPRARELRISRGFDLASFNPHGISTFIDNDDTVYLFVVNHPEFKNTVEIFKFEEGENSLLHLKTVKHELLPSVNDITAVGPAHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPNEVKVVYLCC Predict reactive species Full Name: paraoxonase 2 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC046160 Gene Symbol: PON2 Gene ID (NCBI): 5445 RRID: AB_2166330 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15165 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.