Proteintech, 14435-1-AP, PPME1 Polyclonal antibody

Size: 20ul / 150ul The PPME1 (14435-1-AP) by Proteintech is a Polyclonal antibody targeting PPME1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 14435-1-AP targets PPME1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, fetal human brain tissue, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse brain tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:4000-1:20000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5817 Product name: Recombinant human PPME1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-386 aa of BC050705 Sequence: MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC Predict reactive species Full Name: protein phosphatase methylesterase 1 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC050705 Gene Symbol: PPME1 Gene ID (NCBI): 51400 RRID: AB_2284364 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y570 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.