Proteintech, 14587-1-AP, CES3 Polyclonal antibody

Size: 20ul / 150ul The CES3 (14587-1-AP) by Proteintech is a Polyclonal antibody targeting CES3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 14587-1-AP targets CES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is expressed in liver, colon and small intestine. This antibody recognizes both isoforms with MW 60-62 kDa and 24 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6113 Product name: Recombinant human CES3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 219-568 aa of BC053670 Sequence: DLNCVTVFGGSAGGSIISGLVLSPVAAGLFHRAITQSGVITTPGIIDSHPWPLAQKIANTLACSSSSPAEMVQCLQQKEGEELVLSKKLKNTIYPLTVDGTVFPKSPKELLKEKPFHSVPFLMGVNNHEFSWLIPRGWGLLDTMEQMSREDMLAISTPVLTSLDVPPEMMPTVIDEYLGSNSDAQAKCQAFQEFMGDVFINVPTVSFSRYLRDSGSPVFFYEFQHRPSSFAKIKPAWVKADHGAEGAFVFGGPFLMDESSRLEATEEEKQLSLTMMAQWTHFARTGDPNSKALPPWPQFNQAEQYLEINPVPRAGQKFREAWMQFWSETLPSKIQQWHQKQKNRKAQEDL Predict reactive species Full Name: carboxylesterase 3 Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 60-62 kDa GenBank Accession Number: BC053670 Gene Symbol: CES3 Gene ID (NCBI): 23491 RRID: AB_2275992 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UWW8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.