Proteintech, 14615-1-AP, KIF17 Polyclonal antibody

Size: 20ul / 150ul The KIF17 (14615-1-AP) by Proteintech is a Polyclonal antibody targeting KIF17 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, monkey samples 14615-1-AP targets KIF17 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human gliomas tissue, human brain tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: COS-7 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210). Specification Tested Reactivity: human, mouse, rat, monkey Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6217 Product name: Recombinant human KIF17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC065927 Sequence: MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYLEIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRSHSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDGRCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALLR Predict reactive species Full Name: kinesin family member 17 Calculated Molecular Weight: 115 kDa Observed Molecular Weight: 170 kDa GenBank Accession Number: BC065927 Gene Symbol: KIF17 Gene ID (NCBI): 57576 RRID: AB_2131278 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2E2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.