Proteintech, 14633-1-AP, RPL13A Polyclonal antibody

Size: 20ul / 150ul The RPL13A (14633-1-AP) by Proteintech is a Polyclonal antibody targeting RPL13A in WB, IHC, ELISA applications with reactivity to human, mouse samples 14633-1-AP targets RPL13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Jurkat cells, HEK-293 cells, HeLa cells, RAW264.7 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Ribosomal protein L13a(RPL13a) is a member of the L13 ribosomal protein family and a structural component of the large 60S ribosomal subunit. It's a reference gene(RG) that stably expressed not only during differentiation, but also during expansion to higher passages. And it's suggested as a RG for a variety research field. RPL13a is a key component of the IFN-gamma-activated inhibitor of translation(GAIT) complex, which inhibits translation of a select group of inflammation-related transcripts via binding to rhe 3' UTR of target mRNA.once phosphorylated of Ser77 by ZIPK, RPL13a released from the complex. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6212 Product name: Recombinant human RPL13A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC065236 Sequence: MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV Predict reactive species Full Name: ribosomal protein L13a Calculated Molecular Weight: 23 kDa Observed Molecular Weight: 25-28 kDa GenBank Accession Number: BC065236 Gene Symbol: RPL13A Gene ID (NCBI): 23521 RRID: AB_10638610 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P40429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.