Proteintech, 14648-1-AP, NCF4 Polyclonal antibody

Size: 20ul / 150ul The NCF4 (14648-1-AP) by Proteintech is a Polyclonal antibody targeting NCF4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14648-1-AP targets NCF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: L02 cells Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6241 Product name: Recombinant human NCF4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC002798 Sequence: MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKSVAWEGGACPAFLPSLRPPPLTSPSHGSLSHSKAPSGSQMSHNAVTSHQRPGWPGQPHSPFPHPTPHFQPDASLLQPVTPLGTSRWRKISAALPY Predict reactive species Full Name: neutrophil cytosolic factor 4, 40kDa Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC002798 Gene Symbol: NCF4 Gene ID (NCBI): 4689 RRID: AB_2251237 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15080 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.