Proteintech, 14703-1-AP, SEC6 Polyclonal antibody

Size: 20ul / 150ul The SEC6 (14703-1-AP) by Proteintech is a Polyclonal antibody targeting SEC6 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14703-1-AP targets SEC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, COLO 320 cells, HepG2 cells, human brain tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6383 Product name: Recombinant human SEC6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 381-745 aa of BC064569 Sequence: DTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYYQTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYMIAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTKKWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKMVREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK Predict reactive species Full Name: exocyst complex component 3 Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 86 kDa GenBank Accession Number: BC064569 Gene Symbol: SEC6 Gene ID (NCBI): 11336 RRID: AB_2101542 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60645 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.