Proteintech, 14758-1-AP, AARE Polyclonal antibody

Size: 20ul / 150ul The AARE (14758-1-AP) by Proteintech is a Polyclonal antibody targeting AARE in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 14758-1-AP targets AARE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, human plasma tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6511 Product name: Recombinant human APEH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 381-732 aa of BC000362 Sequence: AQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQEEHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWLRTHLGS Predict reactive species Full Name: N-acylaminoacyl-peptide hydrolase Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 66-80 kDa GenBank Accession Number: BC000362 Gene Symbol: APEH Gene ID (NCBI): 327 RRID: AB_2057811 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13798 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.