Proteintech, 14847-1-AP, CRISP3 Polyclonal antibody

Size: 20ul / 150ul The CRISP3 (14847-1-AP) by Proteintech is a Polyclonal antibody targeting CRISP3 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 14847-1-AP targets CRISP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human saliva, human placenta tissue, Jurkat cells, mouse colon tissue, mouse ovary tissue, mouse thymus tissue Positive IP detected in: human placenta tissue Positive IHC detected in: human prostate cancer tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cysteine-rich secretory protein 3 Calculated Molecular Weight: 28 kDa Observed Molecular Weight: 29 kDa GenBank Accession Number: NM_006061 Gene Symbol: CRISP3 Gene ID (NCBI): 10321 RRID: AB_1607510 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P54108 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.