Proteintech, 14905-1-AP, PSMC2 Polyclonal antibody

Size: 20ul / 150ul The PSMC2 (14905-1-AP) by Proteintech is a Polyclonal antibody targeting PSMC2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14905-1-AP targets PSMC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human adrenal gland tissue, C6 cells, mouse liver tissue, mouse spleen tissue Positive IP detected in: mouse spleen tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6700 Product name: Recombinant human PSMC2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 84-433 aa of BC002589 Sequence: KQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN Predict reactive species Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 2 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC002589 Gene Symbol: PSMC2 Gene ID (NCBI): 5701 RRID: AB_2172484 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35998 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.