Proteintech, 14914-1-AP, HN1 Polyclonal antibody

Size: 20ul / 150ul The HN1 (14914-1-AP) by Proteintech is a Polyclonal antibody targeting HN1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 14914-1-AP targets HN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human brain tissue, human testis tissue, HepG2 cells, A431 cells, LNCaP cells, mouse testis tissue, mouse colon tissue Positive IP detected in: MCF-7 cells Positive IHC detected in: human ovary tumor tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6712 Product name: Recombinant human HN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC001420 Sequence: MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG Predict reactive species Full Name: hematological and neurological expressed 1 Calculated Molecular Weight: 11 kDa, 16 kDa, 19 kDa Observed Molecular Weight: 23-30 kDa GenBank Accession Number: BC001420 Gene Symbol: HN1 Gene ID (NCBI): 51155 RRID: AB_10863118 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UK76 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.