Proteintech, 14921-1-AP, THUMPD1 Polyclonal antibody

Size: 20ul / 150ul The THUMPD1 (14921-1-AP) by Proteintech is a Polyclonal antibody targeting THUMPD1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14921-1-AP targets THUMPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, MDA-MB-231 cells, T-47D cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information THUMPD1, a THUMP domain-containing RNA-binding protein, partners with NAT10 to mediate N4-acetylcytidine (ac4C) modification, enhancing RNA stability, export, and translation. Dysregulated THUMPD1 promotes proliferation, migration, invasion, and EMT. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6739 Product name: Recombinant human THUMPD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-353 aa of BC000448 Sequence: MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGDDMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNREEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSKQGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDFS Predict reactive species Full Name: THUMP domain containing 1 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC000448 Gene Symbol: THUMPD1 Gene ID (NCBI): 55623 RRID: AB_2271785 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NXG2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.