Proteintech, 14941-1-AP, BLMH Polyclonal antibody

Size: 20ul / 150ul The BLMH (14941-1-AP) by Proteintech is a Polyclonal antibody targeting BLMH in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14941-1-AP targets BLMH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human colon tissue, mouse pancreas tissue, mouse spleen tissue Positive IP detected in: mouse skin tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6888 Product name: Recombinant human BLMH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 128-455 aa of BC003616 Sequence: EDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFRVVCICLGNPPETFTWEYRDKDKNYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRPQHKYNKLYTVEYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNSKLGLSDMNLYDHELVFGVSLKNMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALAE Predict reactive species Full Name: bleomycin hydrolase Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC003616 Gene Symbol: BLMH Gene ID (NCBI): 642 RRID: AB_2064803 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13867 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.