Proteintech, 14949-1-AP, ARIH1 Polyclonal antibody

Size: 20ul / 150ul The ARIH1 (14949-1-AP) by Proteintech is a Polyclonal antibody targeting ARIH1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 14949-1-AP targets ARIH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells Positive IHC detected in: mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6760 Product name: Recombinant human ARIH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 209-507 aa of BC051877 Sequence: MQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSG Predict reactive species Full Name: ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 64 kDa GenBank Accession Number: BC051877 Gene Symbol: ARIH1 Gene ID (NCBI): 25820 RRID: AB_3085447 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4X5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.