Proteintech, 14973-1-AP, MTMR14 Polyclonal antibody

Size: 20ul / 150ul The MTMR14 (14973-1-AP) by Proteintech is a Polyclonal antibody targeting MTMR14 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14973-1-AP targets MTMR14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue Positive IHC detected in: human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6883 Product name: Recombinant human MTMR14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 245-598 aa of BC050626 Sequence: LSIPYPGCEFFKEYKDRDYMAEGLIFNWKQDYVDAPLSIPDFLTHSLNIDWSQYQCWDLVQQTQNYLKLLLSLVNSDDDSGLLVHCISGWDRTPLFISLLRLSLWADGLIHTSLKPTEILYLTVAYDWFLFGHMLVDRLSKGEEIFFFCFNFLKHITSEEFSALKTQRRKSLPARDGGFTLEDICMLRRKDRGSTTSLGSDFSLVMESSPGATGSFTYEAVELVPAGAPTQAAWRKSHSSSPQSVLWNRPQPSEDRLPSQQGLAEARSSSSSSSNHSDNFFRMGSSPLEVPKPRLAALSDRETRLQEVRSAFLAAYSSTVGLRAVAPSPSGAIGGLLEQFARGVGLRSISSNAL Predict reactive species Full Name: myotubularin related protein 14 Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 60-72 kDa GenBank Accession Number: BC050626 Gene Symbol: MTMR14 Gene ID (NCBI): 64419 RRID: AB_2147828 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NCE2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.