Proteintech, 15006-1-AP, ARIH2 Polyclonal antibody

Size: 20ul / 150ul The ARIH2 (15006-1-AP) by Proteintech is a Polyclonal antibody targeting ARIH2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15006-1-AP targets ARIH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue Positive IP detected in: HeLa cells Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6507 Product name: Recombinant human ARIH2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 140-493 aa of BC000422 Sequence: AVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT Predict reactive species Full Name: ariadne homolog 2 (Drosophila) Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC000422 Gene Symbol: ARIH2 Gene ID (NCBI): 10425 RRID: AB_2290024 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95376 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.