Proteintech, 15097-1-AP, ATP6V1B2 Polyclonal antibody

Size: 20ul / 150ul The ATP6V1B2 (15097-1-AP) by Proteintech is a Polyclonal antibody targeting ATP6V1B2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 15097-1-AP targets ATP6V1B2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, A549 cells, HeLa cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, K-562 cells, L02 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7174 Product name: Recombinant human ATP6V1B2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 162-511 aa of BC003100 Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH Predict reactive species Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 56-58 kDa GenBank Accession Number: BC003100 Gene Symbol: ATP6V1B2 Gene ID (NCBI): 526 RRID: AB_2243297 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P21281 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.