Proteintech, 15111-1-AP, NSDHL Polyclonal antibody

Size: 20ul / 150ul The NSDHL (15111-1-AP) by Proteintech is a Polyclonal antibody targeting NSDHL in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15111-1-AP targets NSDHL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human heart tissue, mouse brain tissue Positive IHC detected in: human skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3200 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7097 Product name: Recombinant human NSDHL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC000245 Sequence: MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYL Predict reactive species Full Name: NAD(P) dependent steroid dehydrogenase-like Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa, 40 kDa GenBank Accession Number: BC000245 Gene Symbol: NSDHL Gene ID (NCBI): 50814 RRID: AB_2155681 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15738 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.