Proteintech, 15125-1-AP, OGG1 Polyclonal antibody

Size: 20ul / 150ul The OGG1 (15125-1-AP) by Proteintech is a Polyclonal antibody targeting OGG1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15125-1-AP targets OGG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, Transfected HEK-293 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa). Our OGG1 antibody detects all the isoforms. We always got the strongest 47 kDa corresponds to isoform Beta in our detection and some weaker bands (with long time exposure). The expression amount of Beta is higher than other isoforms from our data. This antibody has been cited in more than 4 publications, WB and IHC detection in mouse and human. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7204 Product name: Recombinant human OGG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC000657 Sequence: MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG Predict reactive species Full Name: 8-oxoguanine DNA glycosylase Calculated Molecular Weight: 22 kDa, 36-40 kDa, 45-57 kDa Observed Molecular Weight: 36-40 kDa, 45-50 kDa GenBank Accession Number: BC000657 Gene Symbol: OGG1 Gene ID (NCBI): 4968 RRID: AB_2156780 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15527 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.