Proteintech, 15217-1-AP, RPB5 Polyclonal antibody

Size: 20ul / 150ul The RPB5 (15217-1-AP) by Proteintech is a Polyclonal antibody targeting RPB5 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 15217-1-AP targets RPB5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, HepG2 cells, rat liver tissue, rat spleen tissue, mouse spleen tissue, mouse liver tissue, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. polymerase (RNA) II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E (Polymerase (RNA) II (DNA directed) polypeptide E) /RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. The observed molecular weight of POLR2E is about 25 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7386 Product name: Recombinant human RNA Polymerase II protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC004441 Sequence: MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 23-25 kDa GenBank Accession Number: BC004441 Gene Symbol: RPB5 Gene ID (NCBI): 5434 RRID: AB_2299932 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19388 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.