Proteintech, 15272-1-AP, RIBC2 Polyclonal antibody

Size: 20ul / 150ul The RIBC2 (15272-1-AP) by Proteintech is a Polyclonal antibody targeting RIBC2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15272-1-AP targets RIBC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: fetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse testis tissue, human cervical cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RIBC2 is a microtubule protein and is part of the double microtubules (DMTs) decorated with dynamic proteins in the ciliary axons. It is mainly located in the microtubules of cilia and is involved in flagellated sperm motility(PMID: 40265983). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7385 Product name: Recombinant human RIBC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC003024 Sequence: MRQNDKIMCILENRKKRDRKNLCRAINDFQQSFQKPETRREFDLSDPLALKKDLPARQSDNDVRNTISGMQKFMGEDLNFHERKKFQEEQNREWSLQQQREWKNARAEQKCAEALYTETRLQFDETAKHLQKLESTTRKAVCASVKDFNKSQAIESVERKKQEKKQEQEDNLAEITNLLRGDLLSENPQQAASSFGPHRVVPDRWKGMTQEQLEQIRLVQKQQIQEKLRLQEEKRQRDLDWDRRRIQGARATLLFERQQWRRQRDLRRALDSSNLSLAKEQHLQKKYMNEVYTNQPTGDYFTQFNTGSR Predict reactive species Full Name: RIB43A domain with coiled-coils 2 Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC003024 Gene Symbol: RIBC2 Gene ID (NCBI): 26150 RRID: AB_2179714 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H4K1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.