Proteintech, 15331-1-AP, MVD Polyclonal antibody

Size: 20ul / 150ul The MVD (15331-1-AP) by Proteintech is a Polyclonal antibody targeting MVD in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15331-1-AP targets MVD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, rat liver tissue, HepG2 cells, K-562 cells Positive IHC detected in: human colon tissue, human lung cancer tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7255 Product name: Recombinant human MVD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC000011 Sequence: MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA Predict reactive species Full Name: mevalonate (diphospho) decarboxylase Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 66-74 kDa, 45 kDa, 37 kDa GenBank Accession Number: BC000011 Gene Symbol: MVD Gene ID (NCBI): 4597 RRID: AB_2147439 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P53602 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.