Proteintech, 15387-1-AP, RPL6 Polyclonal antibody

Size: 20ul / 150ul RPL6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA 15387-1-AP targets RPL6 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6840 Product name: Recombinant human RPL6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-288 aa of BC004138 Sequence: MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF Predict reactive species Full Name: ribosomal protein L6 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC004138 Gene Symbol: RPL6 Gene ID (NCBI): 6128 RRID: AB_2182042 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q02878 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.