Proteintech, 15442-1-AP, GMDS Polyclonal antibody

Size: 20ul / 150ul The GMDS (15442-1-AP) by Proteintech is a Polyclonal antibody targeting GMDS in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15442-1-AP targets GMDS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse pancreas tissue, HeLa cells, rat spleen tissue, rat pancreas tissue Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7319 Product name: Recombinant human GMDS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-372 aa of BC000117 Sequence: MAHAPARCPSARGSGDGEMGKPRNVALITGITGQDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVAFDELVREMVHADVELMRTNPNA Predict reactive species Full Name: GDP-mannose 4,6-dehydratase Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 40-42 kDa GenBank Accession Number: BC000117 Gene Symbol: GMDS Gene ID (NCBI): 2762 RRID: AB_2110816 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60547 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.