Proteintech, 15461-1-AP, RPP25 Polyclonal antibody

Size: 20ul / 150ul The RPP25 (15461-1-AP) by Proteintech is a Polyclonal antibody targeting RPP25 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15461-1-AP targets RPP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells Positive IP detected in: HeLa cells Positive IHC detected in: human small intestine tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7742 Product name: Recombinant human RPP25 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-199 aa of BC002497 Sequence: MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA Predict reactive species Full Name: ribonuclease P/MRP 25kDa subunit Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC002497 Gene Symbol: RPP25 Gene ID (NCBI): 54913 RRID: AB_2878142 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BUL9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.