Proteintech, 15491-1-AP, OSBPL10 Polyclonal antibody

Size: 20ul / 150ul The OSBPL10 (15491-1-AP) by Proteintech is a Polyclonal antibody targeting OSBPL10 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15491-1-AP targets OSBPL10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7861 Product name: Recombinant human OSBPL10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-348 aa of BC003168 Sequence: MERAVQGTDGGGGSNSSSRSSSRATSAGSSPSCSLAGRGVSSRSAAAGLGGGGSRSSPGSVAASPSGGGGRRREPALEGVLSKYTNLLQGWQNRYFVLDFEAGILQYFVNEQSKHQKPRGVLSLSGAIVSLSDEAPHMLVVYSANGEMFKLRAADAKEKQFWVTQLRACAKYHMEMNSKSAPSSRSRSLTLLPHGTPNSASPCSQRHLSVGAPGVVTITHHKSPAAARRAKSQYSGQLHEVREMMNQVEGQQKNLVHAIESLPGSGPLTALDQDLLLLKATSAATLSCLGECLNLLQQSVHQAGQPSQKPGASENILGWHGSKSHSTEQLKNGTLGSLPSASANITWA Predict reactive species Full Name: oxysterol binding protein-like 10 Calculated Molecular Weight: 84 kDa Observed Molecular Weight: 75-80 kDa GenBank Accession Number: BC003168 Gene Symbol: OSBPL10 Gene ID (NCBI): 114884 RRID: AB_2158356 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXB5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.